Rob Rouse got his first break in comedy by winning the So You Think You're Funny? new act competition in 1998. As well as stand-up, he also performed as part of the Big And Daft trio with Ian Boldsworth and Jon Williams from 1997 to 2002, taking three shows to the Edinburgh Fringe. In 2002 he took his first solo show to the Fringe.

TV credits include presenting the first series of The Friday Night Project on Channel 4, the C4 sketch show Spoons, and playing Mike in the first series of the BBC3 sitcom Grown Ups.

In 2016, he played Bottom in Ben Elton's Upstart Crow.